I am using TFS 2010. When I created a new project in the Team Project Settings> Source Control checkbox "Enable multiple check-out" on by default. How to disable checkbox "Enable multiple check-out" for new projects?
You need to edit your Process Template.
At first, download it locally by selecting in VS "Team" > "Team Project Collection Settings" > "Process Template Manager...", then click on your template & select Download. This will send all the files on you local PC.
Now open the local file "..\VersionControl\VersionControl.xml" and change entry <exclusive_checkout required="false" /> into true.
Return to "Team" > "Team Project Collection Settings" > "Process Template Manager..." and now select Upload.
Once this is done any new Team Project you generate will have this option "Enable multiple check-out" unset by default.
来源：https://stackoverflow.com/questions/8519495/how-to-disable-checkbox-enable-multiple-check-out-for-new-projects