I am trying to create a plot title manually formatted on two lines which includes two italicized words, I have done some s
We could also call cowplot::draw_label() two times (inspired from this discussion). However, we need to tweak a bit the position and make enough space for the custom title. I gave more explanations about this approach and also using ggplot2::annotation_custom() in ggplot2 two-line label with expression.
library(ggplot2)
library(cowplot)
#>
#> Attaching package: 'cowplot'
#> The following object is masked from 'package:ggplot2':
#>
#> ggsave
# The two lines we wish on the plot. The ~~ creates extra space between the
# expression's components, might be needed here.
line_1 <- expression("First Line of Title with" ~~ italic("Species"))
line_2 <- expression(italic("Species") ~~ "second line words" ~~ italic("anotherSpecies") ~~ "the end")
# Make enough space for the custom two lines title
p <- ggplot(mtcars, aes(x = wt, y = mpg)) +
geom_point() +
labs(title = "") + # empty title
# Force a wider top margin to make enough space
theme(plot.title = element_text(size = 10, # also adjust text size if needed
margin = margin(t = 10, r = 0, b = 0, l = 0,
unit = "mm")))
# Call cowplot::draw_label two times to plot the two lines of text
ggdraw(p) +
draw_label(line_1, x = 0.55, y = 0.97) +
draw_label(line_2, x = 0.55, y = 0.93)

Created on 2019-01-14 by the reprex package (v0.2.1)
As an alternative to inserting line breaks in title, you may use title together with subtitle (available from ggplot 2.2.0). Possibly this makes the plothmathing slightly more straightforward.
p <- ggplot(mtcars, aes(x = wt, y = mpg)) +
geom_point() +
labs(title = expression("First line: "*italic("Honorificabilitudinitatibus")),
subtitle = expression("Second line: "*italic("Honorificabilitudinitatibus praelongus")*" and more"))
p
If you wish the font size to be the same on both lines, set the desired size in theme.
p + theme(plot.title = element_text(size = 12),
plot.subtitle = element_text(size = 12))
Note that both title and subtitle are left-aligned by default in ggplot2 2.2.0. The text can be centered by adding hjust = 0.5 to element_text above.
Using a combination of atop, paste, italic and scriptstyle:
ggplot(mtcars, aes(x = wt, y = mpg)) +
geom_point() +
labs(title = ~ atop(paste('First line of title with ',italic("Species")),
paste(scriptstyle(italic("Species")),
scriptstyle(" secondline words "),
scriptstyle(italic("anotherSpecies")),
scriptstyle(" the end"))))
gives you the desired result:
Using scriptstyle is not a necessity, but imho it is nicer to have your subtitle in a smaller font than the main title.
See also ?plotmath for other usefull customizations.