Here are few which I think are worth knowing but might not be useful on an everyday basis.
Most of them are one liners.
Removing Duplicates from a List
L = list(set(L))
Getting Integers from a string (space seperated)
ints = [int(x) for x in S.split()]
Finding Factorial
fac=lambda(n):reduce(int.__mul__,range(1,n+1),1)
Finding greatest common divisor
>>> def gcd(a,b):
... while(b):a,b=b,a%b
... return a