I have some problems with ggplot legends, here is my first code with only the legend for corrGenes, which is fine.
gene1=c(1.041,0.699,0.602,0.602,2.585,0.60
Finally, I found anther way using a trick. First, I've computed the linear regression and convert the results to a data frame which I add my best fit (Intercept = 0 and slope =1), then I added a column for type of data (data or best).
modele = lm(BIME ~ gene1, data=DF)
coefs = data.frame(intercept=coef(modele)[1],slope=coef(modele)[2])
coefs= rbind(coefs,list(0,1))
regression=as.factor(c('data','best'))
coefs=cbind(coefs,regression)
then I plotted it with a unique geom_abline command and moving the DF from ggplot() to geom_point() and used the linetype parameter to differenciate the two lines :
plot = ggplot()+
geom_point(data=pointSameStrandDF,aes(x=gene1,y=BIME,colour=corrGenes),size=5)+
geom_abline(data=coefs, aes(intercept=intercept,slope=slope,linetype=regression), show_guide=TRUE)+
ylab("BIME normalized counts (log10(RPKM))")+
xlab("gene1 normalized counts (log10(RPKM))")
There is maybe a way to use colors for those 2 lines, but I can't find out how?
Thanks for your help guys!