I am trying to get a FASTA file form NCBI website, I use the following function
getncbiseq <- function(accession){
dbs <- c()
for (i in 1:numdbs){
Thanks for great help. I was stuck at this point for a full day. I finally got the following code worked under windows 10 with R3.4.0(32bits):-
getncbiseq <- function(accession)
{
require("seqinr") # this function requires the SeqinR R package
# first find which ACNUC database the accession is stored in:
dbs <- c("genbank","refseq","refseqViruses","bacterial")
numdbs <- length(dbs)
for (i in 1:numdbs)
{
db <- dbs[i]
choosebank(db)
# check if the sequence is in ACNUC database 'db':
resquery <- try(query(".tmpquery", paste("AC=", accession)), silent = TRUE)
if (!(inherits(resquery, "try-error"))) {
queryname <- "query2"
thequery <- paste("AC=", accession, sep="")
query2 <- query(queryname, thequery)
# see if a sequence was retrieved:
seq <- getSequence(query2$req[[1]])
closebank()
return(seq)
}
closebank()
}
print(paste("ERROR: accession",accession,"was not found"))
}