My MVC app has, until a few minutes ago, been working fine (with asp/net membership as part of the solution). However, without knowingly changing anything relevant every req
In applicationhost.config check if there is any entry as shown below. If there is any such entry change anonymousAuthetication enabled value from 'false' to 'true'.
Option-2:
If you are using visual studio, make sure that anonymousAuthentication is enabled.