For clarification: I know how evil globals are and when not to use them :)
There is no performance penalty, either way, that you should be concerned about. In adition to what everyone else has said, you should also bear in mind the paging overhead. Local instance variables are fetched from the object structure which has likely (?) already been paged into cache memory). Global variables, on the other hand, may cause a different pattern of virtual memory paging.
Again, the performance is really not worth any consideration on your part.