My practice is to use two main pages, let's say index.py and ajax.py. First one is responsible for generating full website, and is default target of forms. Other one generates only output specific for adequate ajax query. Logic behind both of them is the same, only the method of generating output is a bit different.
In jquery I simply change action parameter when sending a request. It works both with and without ajax, although long time have I not seen someone with disabled js and ajax.
