I use the following sed command to remove the line number string from file
sed -i s\'/line number//g\' file
but what I need to
I'm not sure I fully understand your question, however this can help you :
sed -i '$ s/line number//g' file
This will perform substitution only on last line (because of $), replacing line number by nothing.
man sed, section Addresses can help you understand how to apply sed commands on part of the text.