marc_s is right with his comment. What you need is a function like in this answer.
You would then call it like this:
SELECT
udfTimeSpanFromSeconds(SUM(DATEDIFF(SECOND, t.Paydate, t.DelDate))) AS 'HH:MM:SS'
FROM
Transaction_tbl t
WHERE
t.transactID in (24, 25)
group by
vtid