I have a database test.db with the first column containing 123456;abcdef;ghijk etc. Is it possible to split the data into its own colums?
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There is no built-in SQLite function that can split strings like this.
If your are using the SQLite C API or a wrapper like APSW, you could create your own function (C, APSW).
If you want to do nothing more than a one-time conversion, export/import through a text file would be the simplest solution.