How can i make layout like this using CSS stylesheet for GTK app?
Here is example code:
#!/usr/bin/py
Not right now. GTK doesn't support margin properties on its widgets, and it only supports padding properties on widgets that draw a frame. (Which elements draw a frame can be a bit arbitrary, but Gtk.Box and Gtk.Label don't, so that's why your example doesn't work. You can fake it on any widget by putting it inside a Gtk.Frame though.)
This blog post reveals that margin and padding are planned to be supported consistently on all widgets in the upcoming GTK 3.20.