Trying to validate a comma-separated email list in the textbox with asp:RegularExpressionValidator, see below:
asp:RegularExpressionValidator
The regex below is less restrictive and more appropriate for validating a manually-entered list of comma-separated email addresses. It allows for adjacent commas.
^([\w+-.%]+@[\w-.]+\.[A-Za-z]{2,4},*[\W]*)+$