Trying to validate a comma-separated email list in the textbox with asp:RegularExpressionValidator, see below:
Try this:
^([\w+-.%]+@[\w-.]+\.[A-Za-z]{2,4},?)+$
Adding the + after the parentheses means that the preceding group can be present 1 or more times.
Adding the ^ and $ means that anything between the start of the string and the start of the match (or the end of the match and the end of the string) causes the validation to fail.