I get a 404 HTTP status error (not found) on a specific WebResource.axd call inside an ASP.NET 3.5 (AJAX) web application. I guess the erro
Is your project missing any references?
Are there any references set to CopyLocal=False (common with Infragistics or GAC'ed refs) that dont make it to the destination?
A utility like reflector or dependency walker will tell you if your main assembly is missing any dependencies that are not immediately obvious.
Does the Application_Error handler in global.asax have a catch that is producing any error info (FileNotFoundExceptions)?
Did you set custom errors to 'remote only' and browse the site from the local machine?