This will work, but I don't know if you want to retain the items in the list:
max1 = max(myList)
myList.remove(max1)
max2 = max(myList)
If you do, you can do this:
max1 = max(myList)
idx1 = myList.index(max1)
myList.pop(idx1)
max2 = max(myList)
myList.insert(idx1,max1)