I was shocked when the following code did not do what I expected it to do:
lines_list = [\'this is line 1\\n\', \'this is line 2\\n\', \'this is line 3\\n\']
You did forget to assigned new value (stripped string) to something.
Another way, how to assign it to a list in shorter way is:
>>> lst = ['this is line 1\n', 'this is line 2\n', 'this is line 3\n']
>>> import string
>>> lst = map(string.strip, lst)
>>> lst
['this is line 1', 'this is line 2', 'this is line 3]