Hello how can i remove item from generic list here is my code im trying to do it right but i dont know where i make mistake;/
Users us_end = new Users();
for
You are creating a new Users object - this is not the same as any object already in Application["Users_On"] (it will have a different reference), so it will not be removed.
This assumes that Equals and/or IEquatable were not overridden/implemented in Users.
List us = ((List)Application["Users_On"]);
Users us_end = us.Where(u => u.Id == (int)Session["Current_Id"]).FirstOrDefault();
us.Remove(us_end);
Application["Users_On"] = us;
By the way - your variable naming is not very good - go for more descriptive names.