The question of how to speed up importing of Python modules has been asked previously (Speeding up the python "import" loader and Python -- Speed Up Imports?) but
1.35 seconds isn't long, but I suppose if you're used to half that for a "quick check" then perhaps it seems so.
Andrea suggests a simple client/server setup, but it seems to me that you could just as easily call a very slight modification of your script and keep it's console window open while you work:
I assume your script is identical every time, ie you don't need to give it image stack location or any particular commands each time (but these are easy to do as well!).
Example RAAC's_Script.py:
import numpy as np
import matplotlib.pyplot as plt
import matplotlib.animation as animation
import scipy.ndimage
import scipy.signal
import sys
import os
print('********* RAAC\'s Script Now Running *********')
while True: # Loops forever
# Display a message and wait for user to enter text followed by enter key.
# In this case, we're not expecting any text at all and if there is any it's ignored
input('Press Enter to test image stack...')
'''
*
*
**RAAC's Code Goes Here** (Make sure it's indented/inside the while loop!)
*
*
'''
To end the script, close the console window or press ctrl+c.
I've made this as simple as possible, but it would require very little extra to handle things like quitting nicely, doing slightly different things based on input, etc.