I am trying to convert a bootloader I wrote in Assembly Language to an ISO image file. The following is the code from MikeOS bootloader. Here is my bootloader code:
For CD; there's a specification ("El Torito") that describes how bootable CDs work; where the first 16 (2048-byte) sectors are unused, there's a "boot catalogue" that the firmware uses to decide which boot loader it should use (so you can have a single CD that boots very different systems - e.g. PC BIOS, UEFI, PowerPC, etc), then the boot loaders themselves.
For "PC BIOS" alone, there's 3 possibilities:
The first 2 options are mostly for compatibly purposes only (crusty old OSs that don't support booting from CDs, like MS-DOS); and have performance implications (e.g. to emulate loading one 512-byte virtual sector, the firmware has to load a real 2048-byte sector and throw away the excess 1536 bytes). Any OS designed/written in the last 15+ years should be using "no emulation".
For "no emulation":
Also note that (in general) for "PC BIOS" you're probably going to want 5 different boot loaders (one for floppy, one for "MBR partitioned" hard disk, one for "GPT partitioned" hard disk, one for CD, and one for network boot). These cases are all different enough (and the "one 512-byte initial sector only" limitation for 3 of these cases is restrictive enough) to make the "all devices supported by one boot loader" idea a disaster.
To actually generate the ISO; you can use an existing tool (e.g. mkisofs), or you can write your own tool (ISO9660 and "El Torito" are both relatively easy to understand, and writing your own tool to generate an ISO can be done in less than 2 days, which is like a drop in the ocean for OS development projects).