How to fix ASP.NET error “The file 'nnn.aspx' has not been pre-compiled, and cannot be requested.”?

Question

I have a VS 2005 web site that I publish using \"Publish Web Site\", and I clear all the three checkboxes. I also have a deployment project that picks up the published files

Answer 1

Just as a footnote to all of the above answers that resolved the issue via republishing to replace a missing assembly... While i have solved this issue previously with that same solution, i have just encountered another reason for it's occurrence which may assist others.

The AppPool that my site was running under had it's "Enable 32-bit applications" setting set to false. By changing this to true via the "Advanced Settings" dialog of the app pool i resolved my issue.

Hope that helps some other poor sucker.