I have noticed that the first time I run a script, it takes considerably more time than the second and third time1. The \"warm-up\" is mentioned in this question
Another issue not mensioned by Amro and Marc is memory (pre)allocation.
If your script does not pre-allocate its memory it's first run would be very slow due to memory allocation. Once it completed its first iteration all memory is allocated, so consecutive invokations of the script would be more efficient.
An illustrative example
for ii = 1:1000
vec(ii) = ii; %// vec grows inside loop the first time this code is executed only
end