if add row to DataTable
DataRow row = datatable1.NewRow();
row[\"column2\"]=\"column2\";
row[\"column6\"]=\"column6\";
datatable1.Rows.Add(row);
If you need to manipulate anything aside from the Cell Value string such as adding a Tag, try this:
DataGridViewRow newRow = (DataGridViewRow)mappingDataGridView.RowTemplate.Clone();
newRow.CreateCells(mappingDataGridView);
newRow.Cells[0].Value = mapping.Key;
newRow.Cells[1].Value = ((BusinessObject)mapping.Value).Name;
newRow.Cells[1].Tag = mapping.Value;
mappingDataGridView.Rows.Add(newRow);