I have two xlsx files as follows:
xlsx
value1 value2 value3 0.456 3.456 0.4325436 6.24654 0.235435 6.376546 4.26545 4.264543 7.2564523
Use pandas and you can do it as simple as this:
import pandas as pd df1 = pd.read_excel('excel1.xlsx') df2 = pd.read_excel('excel2.xlsx') difference = df1[df1!=df2] print difference
And the result will look like this: