I am sending email to some users and wants to know who had read it, means if some one had read that email then a log file will maintain which contain the email address of th
This is next to impossible to do 100% effectively.
You could control where the content is stored e.g. http://www.example.com/34hg038g85gb8no84g5 and provide a link in the email to that content, you can then detect when that URL was viewed.
Use a method used by MailChimp and other newsletter campaigns, put an invisible image in your email, this image should reside on a server you control, you can then detect when that image is hit when the user opens the email.