I have two scripts, the main is in Python 3, and the second one is written in Python 2 (it also uses a Python 2 library).
There is one method in the Python 2 script
It works for me if I call the python 2 executable directly from a python 3 environment.
python2_command = 'C:\Python27\python.exe python2_script.py arg1'
process = subprocess.Popen(python2_command.split(), stdout=subprocess.PIPE)
output, error = process.communicate()
python3_command = 'python python3_script.py arg1'
process = subprocess.Popen(python3_command.split(), stdout=subprocess.PIPE)
output, error = process.communicate()