I am working on Retinal fundus images.The image consists of a circular retina on a black background. With OpenCV, I have managed to get a contour which surrounds the whole c
Here's another approach to crop out a rectangular ROI. The main idea is to find the edges of the retina using Canny edge detection, find contours, and then extract the ROI using Numpy slicing. Assuming you have an input image like this:

Extracted ROI

import cv2
# Load image, convert to grayscale, and find edges
image = cv2.imread('1.jpg')
gray = cv2.cvtColor(image, cv2.COLOR_BGR2GRAY)
thresh = cv2.threshold(gray, 0, 255, cv2.THRESH_OTSU + cv2.THRESH_BINARY)[1]
# Find contour and sort by contour area
cnts = cv2.findContours(thresh, cv2.RETR_EXTERNAL, cv2.CHAIN_APPROX_SIMPLE)
cnts = cnts[0] if len(cnts) == 2 else cnts[1]
cnts = sorted(cnts, key=cv2.contourArea, reverse=True)
# Find bounding box and extract ROI
for c in cnts:
x,y,w,h = cv2.boundingRect(c)
ROI = image[y:y+h, x:x+w]
break
cv2.imshow('ROI',ROI)
cv2.imwrite('ROI.png',ROI)
cv2.waitKey()