So if I go into QtDesigner and build a UI, it\'ll be saved as a .ui file. How can I make this as a python file or use this in python?
You need to generate a python file from your ui file with the pyuic tool (site-packages\pyqt4\bin)
pyuic form1.ui > form1.py
with pyqt4
pyuic4.bat form1.ui > form1.py
Then you can import the form1 into your script.