Here is a Python 3, POSIX solution (not Windows!) that uses mmap to map the object into memory.
import hashlib
import mmap
def sha256sum(filename):
h = hashlib.sha256()
with open(filename, 'rb') as f:
with mmap.mmap(f.fileno(), 0, prot=mmap.PROT_READ) as mm:
h.update(mm)
return h.hexdigest()