I have a Mathematica expression that contains a single square root, schematically
expr = a / (b + Sqrt[c]);
where a,b
If you are willing to change the assignment to expr, you can do this:
expr = Hold[a / (b + Sqrt[c])];
Cases[expr, HoldPattern @ Sqrt[x_] :> x, Infinity]
The Hold in the assignment statement prevents Mathematica from applying any simplifications to the expression. In this case, Sqrt[c] gets "simplified" into Power[c,Rational[1,2]].
The HoldPattern is essential in the Cases expression to prevent the same simplification from happening to the pattern being matched.