I've been looking around for hours now. The only thing that makes sense to me is http://fnch.users.sourceforge.net/portablepygtkwindows.html But it takes up >40MB of space and it is too big for me.
Other tutorials are either too complicated for me to go through them or the results I obtained didn't work.
I've tried py2exe but the import gtk fails.
I've tried pyinstaller. The GTK files were included but not the glade file.
I would wish to have it all in a single standalone .exe. But now my priority is to have it properly working in windows without Python. Any effort to help is much appreciated.
Some instructions on py2exe and pygtk are available. You should be able to have your whole program in a single exe file, but not the GTK library, which will need to be copied:
Once that's done, you'll need to copy the etc, lib and share directories from your GTK+ install (not the pygtk install) to the dist dir py2exe created.
Looks you can also ship data files with pyinstaller.
来源：https://stackoverflow.com/questions/13799317/converting-pygtk-to-exe